LOCUS       sequence-128510-        1153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      388..397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(394..396,397..399,400..1113)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(439..460)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(700..719)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1047..1068
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgaccgtca gatccgctag cgccaccatg aagttaagtc gccagttcac cgtgtttggc
       61 agcgcgatct tctgcgtcgt aatcttctca ctctacctga tgctggacag gggtcacttg
      121 gactaccctc ggggcccgcg ccaggagggc tcctttccgc agggccagct ttcaatattg
      181 caagaaaaga ttgaccattt ggagcgtttg ctcgctgaga acaacgagat catctcaaat
      241 atcagagact cagtcatcaa cctgagcgag tctgtggagg acggcccgcg ggggtcacca
      301 ggcaacgcca gccaaggctc catccacctc cactcgccac agttggccct gcaggctgac
      361 cccagctccg gatcggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
      421 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      481 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      541 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgag ctggggcgtg
      601 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      661 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      721 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      781 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catccacggc
      841 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt cggcctcaac
      901 tgcaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      961 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     1021 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1081 atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca
     1141 gccataccac att
//
