LOCUS       sequence-128511-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M
  TITLE     Genetically encoded FRET sensors to monitor intracellular Zn(2+) 
            homeostasis.
  JOURNAL   Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
  PUBMED    19718032
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-10"; volume: "6"; issue: 
            "10"; pages: "737-40"
COMMENT     Sequence Label: peZinCh-1
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(378..1088,1089..1091))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATHGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALCKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(420..441)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     769..788
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtgg
       61 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      121 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatgggt
      181 accgagcccc gcggaccccc ggatccaccg ctaccaccgg atccaccgct tccaccggat
      241 ccaccgctac caccggatcc accgcttcca ccggatccgc cgctgccgcc ggatccgccg
      301 gagccgccgg atccgccgct accgccggat ccgccgcttc cgccggatcc gccacgaatg
      361 agctcgccgc ctccggactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
      421 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgca cagggcggac
      481 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      541 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
      601 ttggccttga tgccgttctt ctgcttgtcg gcggtgatat agacgttgtc gctgatggcg
      661 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
      721 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
      781 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
      841 aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcactgcac gccccaggtc
      901 agggtggtca cgagggtggg ccagggcacg ggcagcttac cggtggtgca gatgaacttc
      961 agggtcagct tgccgtgggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     1021 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     1081 cccttgctca c
//
