LOCUS       sequence-128515-        5011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To see HBV S2 promoter activity for luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ko C, Lee S, Windisch MP, Ryu WS
  TITLE     DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis
            B virus replication at the transcriptional level.
  JOURNAL   J Virol. 2014 Dec;88(23):13689-98. doi: 10.1128/JVI.02035-14. Epub 
            2014 Sep 17.
  PUBMED    25231298
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02035-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "23"; pages: "13689-98"
COMMENT     Sequence Label: pHBV-S2-Luc
FEATURES             Location/Qualifiers
     source          1..5011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      275..284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             281..1933
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(347..365)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1974..2095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1985..2004)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2039..2058
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2343..2360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2514..3102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2594..2613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3273..4064,4065..4133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3896..3915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4134..4238)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4265..4720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4352..4371)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4562..4583
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4851..4899
                     /note="synthetic polyadenylation signal"
     misc_feature    4913..5004
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4953..4972
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagaac aaggacacct ggccagacgc
       61 caacaaggta ggagctggag cattcgggct gggtttcacc ccaccgcacg gaggcctttt
      121 ggggtggagc cctcaggctc agggcatact acaaactttg ccagcaaatc cgcctcctgc
      181 ctccaccaat cgccagtcag gaaggcagcc taccccgctg tctccacctt tgagaaacaa
      241 gcttgggtgg tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat
      301 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
      361 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
      421 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
      481 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
      541 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
      601 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
      661 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
      721 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
      781 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
      841 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg
      901 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt
      961 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt
     1021 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag
     1081 atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct
     1141 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc
     1201 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt
     1261 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc
     1321 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc
     1381 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag
     1441 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc
     1501 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga
     1561 cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta
     1621 tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc
     1681 aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt
     1741 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac
     1801 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac
     1861 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa
     1921 gatcgccgtg taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
     1981 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2041 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     2101 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     2161 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga
     2221 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     2281 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
     2341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2701 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2941 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4261 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4321 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4381 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4441 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4501 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4561 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4621 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4681 tttaacgcga attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg
     4741 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
     4801 ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat
     4861 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
     4921 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
     4981 gtgcaggtgc cagaacattt ctctatcgat a
//
