LOCUS       sequence-128528-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standardized mini-Tn5 delivery plasmid, gentamicin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Martinez-Garcia E, Aparicio T, de Lorenzo V, Nikel PI
  TITLE     New transposon tools tailored for metabolic engineering of 
            gram-negative microbial cell factories.
  JOURNAL   Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 
            10.3389/fbioe.2014.00046. eCollection 2014.
  PUBMED    25389526
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2014-10-28"; volume: "2:46. doi"; pages:
            " 10.3389/fbioe.2014.00046. eCollection 2014"
COMMENT     Sequence Label: pBAMD1-6
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(193..198,199..215,216..221))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    268..324
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    358..376
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     terminator      391..477
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
       61 gatgcccgag gcataggctg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
      121 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
      181 tacttgcatt acagtttacg aaccgaacag gcttatgtca aatttaaata cagaaaagcc
      241 cgcctttcgg cgggctttgg cggccgcaag cttgcatgcc tgcaggtcga ctctagagga
      301 tccccgggta ccgagctcga attcgcgcgg ccgcggccta ggcggcctga gacacaaaga
      361 tgtgtataag agacagttaa ttaaaggcat caaataaaac gaaaggctca gtcgaaagac
      421 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
      481 ccgccctaga cagctgggcg cgccccatgt cagccgttaa gtgttcctgt gtcactcaaa
      541 attgctttga gaggctctaa gggcttctca gtgcgttaca tccctggctt gttgtccaca
      601 accgttaaac cttaaaggct ttaaaagcct tatatattct tttttttctt ataaaactta
      661 aaaccttaga ggctatttaa gttgctgatt tatattaatt ttattgttca aacatgagag
      721 cttagtacgt gaaacatgag agcttagtac gttagccatg agagcttagt acgttagcca
      781 tgagg
//
