LOCUS       sequence-128531-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CyclinB-GFP in pIVT vector for in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Davydenko O, Schultz RM, Lampson MA
  TITLE     Increased CDK1 activity determines the timing of 
            kinetochore-microtubule attachments in meiosis I.
  JOURNAL   J Cell Biol. 2013 Jul 22;202(2):221-9. doi: 10.1083/jcb.201303019. 
            Epub 2013 Jul 15.
  PUBMED    23857768
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201303019"; journalName: "J Cell Biol"; date: 
            "2013-07-22- 22"; volume: "202"; issue: "2"; pages: "221-9"
COMMENT     Sequence Label: CyclinB-GFP
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..723)
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDGTYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGIAHGMDELYK"
     primer_bind     complement(53..73)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     668..696
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgcggccgct tatttgtata gttcatccat gccatgtgca atcccagcag ccgttacaaa
       61 ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg cagattgtgt
      121 ggacaggtaa tggttgtctg gtaaaaggac agggtcatcg ccaattggag tattttgttg
      181 ataatgatca gcgagttgca cgccgccgtc ttcgatgttg tggcgggtct tgaagttggc
      241 tttgatgccg ttcttttgct tgtcggccat gatgtatacg ttgtgggagt tgtagttgta
      301 ttccaacttg tggccgagga tgtttccgtc ctccttgaaa tcgattccct taagctcgat
      361 cctgttgacg agggtgtctc cctcaaactt gacttcagca cgtgtcttgt agttcccgtc
      421 gtccttgaag aagatggtcc tctcctgcac gtatccctca ggcatggcgc tcttgaagaa
      481 gtcgtgccgc ttcatatgat ctggatatct tgaaaagcat tgaacaccat aagtgagagt
      541 agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa atttaagggt
      601 aagttttccg tatgttccat caccttcacc ctctccactg acagaaaatt tgtgcccatt
      661 aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt cttctccttt
      721 actgaattgc gctcctgcgt cgaccacctt tgccacagcc ttggctaaat cttgaactag
      781 tgcagaattc agctgtggta gagtgctgat cttagcatgc ttcgatgtgg catacttgtt
      841 cttgacagtc atgtgctttg taagtccttg att
//
