LOCUS       sequence-128533-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A 
            mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Douglas ME, Davies T, Joseph N, Mishima M
  TITLE     Aurora B and 14-3-3 coordinately regulate clustering of 
            centralspindlin during cytokinesis.
  JOURNAL   Curr Biol. 2010 May 25;20(10):927-33. doi: 
            10.1016/j.cub.2010.03.055. Epub 2010 May 6.
  PUBMED    20451386
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2010.03.055"; journalName: "Curr Biol"; date: 
            "2010-05-25- 25"; volume: "20"; issue: "10"; pages: "927-33"
COMMENT     Sequence Label: pEGFP-C1-MKLP1 S708/710A
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     290..311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
       61 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      121 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      181 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      241 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      301 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac
      361 tcagatctcg agctctgggc atggggaagt cagcgagagc taagacaccc cggaaaccta
      421 ccgtgaaaaa agggtcccaa acgaacctta aagacccagt tggggtatac tgtagggtgc
      481 gcccactggg ctttcctgat caagagtgtt gcatagaagt gatcaataat acaactgttc
      541 agcttcatac tcctgagggc tacagactca accgaaatgg agactataag gagactcagt
      601 attcatttaa acaagtattt ggcactcaca ccacccagaa ggaactcttt gatgttgtgg
      661 ctaatccctt ggtcaatgac ctcattcatg gcaaaaatgg tcttcttttt acatatggtg
      721 tgacgggaag tggaaaaact cacacaatga ctggttctcc aggggaagga gggctgcttc
      781 ctcgttgttt ggacatgatc tttaacagta tagggtcatt tcaagctaaa cgatatgttt
      841 tcaaatctaa tgataggaat agtatggata tacagtgtga ggttgatg
//
