LOCUS       sequence-128534-        2919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CyclinB-GFP in pIVT vector for in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Davydenko O, Schultz RM, Lampson MA
  TITLE     Increased CDK1 activity determines the timing of 
            kinetochore-microtubule attachments in meiosis I.
  JOURNAL   J Cell Biol. 2013 Jul 22;202(2):221-9. doi: 10.1083/jcb.201303019. 
            Epub 2013 Jul 15.
  PUBMED    23857768
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201303019"; journalName: "J Cell Biol"; date: 
            "2013-07-22- 22"; volume: "202"; issue: "2"; pages: "221-9"
COMMENT     Sequence Label: CyclinB-GFP
FEATURES             Location/Qualifiers
     source          1..2919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     256..275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     509..526
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    643..664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(679..684,685..702,703..709)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    717..733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     722..744
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     741..757
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     741..757
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        778..796
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           821..863
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2191..2904
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDGTYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGIAHGMDELYK"
     primer_bind     complement(2218..2246)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2841..2861
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
       61 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      421 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      481 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      541 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      601 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      661 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      721 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat
      781 taaccctcac taaagggaac aaaagctgga gctaagcttg cttgttcttt ttgcagaagc
      841 tcagaataaa cgctcaactt tggcagatct atggcgctcc gagtcaccag gaactcgaaa
      901 attaatgctg aaaataaggc gaagatcaac atggcaggcg caaagcgcgt tcctacggcc
      961 cctgctgcaa cctccaagcc cggactgagg ccaagaacag ctcttgggga cattggtaac
     1021 aaagtcagtg aacaactgca ggccaaaatg cctatgaaga aggaagcaga accttcagct
     1081 actggaaaag tcattgataa aaaactacca aaacctcttg aaaaggtacc tatgctggtg
     1141 ccagtgccag tgtctgagcc agtgccagag ccagaacctg agccagaacc tgagcctgtt
     1201 aaagaagaaa aactttcgcc tgagcctatt ttggttgata ctgcctctcc aagcccaatg
     1261 gaaacatctg gatgtgcccc tgcagaagaa gacctgtgtc aggctttctc tgatgtaact
     1321 cttgcagtaa atgatgtgga tgcagaagat ggagctgatc caaacctttg tagtgaatat
     1381 gtgaaagata tttatgctta tctgagacaa cttgaggaag agcaagcagt cagaccaaaa
     1441 tacctactgg gtcgggaagt cactggaaac atgagagcca tcctaattga ctggctagta
     1501 caggttcaaa tgaaattcag gttgttgcag gagaccatgt acatgactgt ctccattatt
     1561 gatcggttca tgcagaacaa ttgtgtgccc aagaagatgc tgcagctggt tggtgtcact
     1621 gccatgttta ttgcaagcaa atatgaagaa atgtaccctc cagaaattgg tgactttgct
     1681 tttgtgactg acaacactta tactaagcac caaatcagac agatggaaat gaagattcta
     1741 agagctttaa actttggtct gggtcggcct ctacctttgc acttccttcg gagagcatct
     1801 aagattggag aggttgatgt cgagcaacat actttggcca aatacctgat ggaactaact
     1861 atgttggact atgacatggt gcactttcct ccttctcaaa ttgcagcagg agctttttgc
     1921 ttagcactga aaattctgga taatggtgaa tggacaccaa ctctacaaca ttacctgtca
     1981 tatactgaag aatctcttct tccagttatg cagcacctgg ctaagaatgt agtcatggta
     2041 aatcaaggac ttacaaagca catgactgtc aagaacaagt atgccacatc gaagcatgct
     2101 aagatcagca ctctaccaca gctgaattct gcactagttc aagatttagc caaggctgtg
     2161 gcaaaggtgg tcgacgcagg agcgcaattc agtaaaggag aagaactttt cactggagtt
     2221 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     2281 gagggtgaag gtgatggaac atacggaaaa cttaccctta aatttatttg cactactgga
     2341 aaactacctg ttccatggcc aacacttgtc actactctca cttatggtgt tcaatgcttt
     2401 tcaagatatc cagatcatat gaagcggcac gacttcttca agagcgccat gcctgaggga
     2461 tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac acgtgctgaa
     2521 gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat cgatttcaag
     2581 gaggacggaa acatcctcgg ccacaagttg gaatacaact acaactccca caacgtatac
     2641 atcatggccg acaagcaaaa gaacggcatc aaagccaact tcaagacccg ccacaacatc
     2701 gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa atactccaat tggcgatgac
     2761 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     2821 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cggctgctgg gattgcacat
     2881 ggcatggatg aactatacaa ataagcggcc gcgatccgc
//
