LOCUS       sequence-128538-        9318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Takagi J, Petre BM, Walz T, Springer TA
  TITLE     Global conformational rearrangements in integrin extracellular 
            domains in outside-in and inside-out signaling.
  JOURNAL   Cell. 2002 Sep 6. 110(5):599-11.
  PUBMED    12230977
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2002 
            Sep 6. 110(5):599-11."
COMMENT     Sequence Label: pEF1-alpha-IIb
FEATURES             Location/Qualifiers
     source          1..9318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(166..185)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        473..1651
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          704..1642
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1599..1619
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1668..1687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1668..1686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4949..4990
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5000..5017
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5040..5057)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5046..5270
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5316..5744
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5403..5422)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5613..5634
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5753..5773)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5758..6087
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5938..6073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6000..6019
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6154..6948
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6208..6227)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6818..6837
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7124..7245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7161..7180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7215..7234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7294..7310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7294..7310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7307..7329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7318..7334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7342..7348,7349..7366,7367..7372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7387..7408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7525..7542)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7685..8273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7765..7784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8444..9235,9236..9304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9067..9086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9305..9318,1..91))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
     1741 tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gtccagtgtg
     1801 gtggaattcc aagatggcca gagctttgtg tccactgcaa gccctctggc ttctggagtg
     1861 ggtgctgctg ctcttgggac cttgtgctgc ccctccagcc tgggccttga acctggaccc
     1921 agtgcagctc accttctatg caggccccaa tggcagccag tttggatttt cactggactt
     1981 ccacaaggac agccatggga gagtggccat cgtggtgggc gccccgcgga ccctgggccc
     2041 cagccaggag gagacgggcg gcgtgttcct gtgcccctgg agggccgagg gcggccagtg
     2101 cccctcgctg ctctttgacc tccgtgatga gacccgaaat gtaggctccc aaactttaca
     2161 aaccttcaag gcccgccaag gactgggggc gtcggtcgtc agctggagcg acgtcattgt
     2221 ggcctgcgcc ccctggcagc actggaacgt cctagaaaag actgaggagg ctgagaagac
     2281 gcccgtaggt agctgctttt tggctcagcc agagagcggc cgccgcgccg agtactcccc
     2341 ctgtcgcggg aacaccctga gccgcattta cgtggaaaat gattttagct gggacaagcg
     2401 ttactgtgaa gcgggcttca gctccgtggt cactcaggcc ggagagctgg tgcttggggc
     2461 tcctggcggc tattatttct taggtctcct ggcccaggct ccagttgcgg atattttctc
     2521 gagttaccgc ccaggcatcc ttttgtggca cgtgtcctcc cagagcctct cctttgactc
     2581 cagcaaccca gagtacttcg acggctactg ggggtactcg gtggccgtgg gcgagttcga
     2641 cggggatctc aacactacag aatatgtcgt cggtgccccc acttggagct ggaccctggg
     2701 agcggtggaa attttggatt cctactacca gaggctgcat cggctgcgcg gagagcagat
     2761 ggcgtcgtat tttgggcatt cagtggctgt cactgacgtc aacggggatg ggaggcatga
     2821 tctgctggtg ggcgctccac tgtatatgga gagccgggca gaccgaaaac tggccgaagt
     2881 ggggcgtgtg tatttgttcc tgcagccgcg aggcccccac gcgctgggtg cccccagcct
     2941 cctgctgact ggcacacagc tctatgggcg attcggctct gccatcgcac ccctgggcga
     3001 cctcgaccgg gatggctaca atgacattgc agtggctgcc ccctacgggg gtcccagtgg
     3061 ccggggccaa gtgctggtgt tcctgggtca gagtgagggg ctgaggtcac gtccctccca
     3121 ggtcctggac agccccttcc ccacaggctc tgcctttggc ttctcccttc gaggtgccgt
     3181 agacatcgat gacaacggat acccagacct gatcgtggga gcttacgggg ccaaccaggt
     3241 ggctgtgtac agagctcagc cagtggtgaa ggcctctgtc cagctactgg tgcaagattc
     3301 actgaatcct gctgtgaaga gctgtgtcct acctcagacc aagacacccg tgagctgctt
     3361 caacatccag atgtgtgttg gagccactgg gcacaacatt cctcagaagc tatccctaaa
     3421 tgccgagctg cagctggacc ggcagaagcc ccgccagggc cggcgggtgc tgctgctggg
     3481 ctctcaacag gcaggcacca ccctgaacct ggatctgggc ggaaagcaca gccccatctg
     3541 ccacaccacc atggccttcc ttcgagatga ggcagacttc cgggacaagc tgagccccat
     3601 tgtgctcagc ctcaatgtgt ccctaccgcc cacggaggct ggaatggccc ctgctgtcgt
     3661 gctgcatgga gacacccatg tgcaggagca gacacgaatc gtcctggact gtggggaaga
     3721 tgacgtatgt gtgccccagc ttcagctcac tgccagcgtg acgggctccc cgctcctagt
     3781 tggggcagat aatgtcctgg agctgcagat ggacgcagcc aacgagggcg agggggccta
     3841 tgaagcagag ctggccgtgc acctgcccca gggcgcccac tacatgcggg ccctaagcaa
     3901 tgtcgagggc tttgagagac tcatctgtaa tcagaagaag gagaatgaga ccagggtggt
     3961 gctgtgtgag ctgggcaacc ccatgaagaa gaacgcccag ataggaatcg cgatgttggt
     4021 gagcgtgggg aatctggaag aggctgggga gtctgtgtcc ttccagctgc agatacggag
     4081 caagaacagc cagaatccaa acagcaagat tgtgctgctg gacgtgccgg tccgggcaga
     4141 ggcccaagtg gagctgcgag ggaactcctt tccagcctcc ctggtggtgg cagcagaaga
     4201 aggtgagagg gagcagaaca gcttggacag ctggggaccc aaagtggagc acacctatga
     4261 gctccacaac aatggccctg ggactgtgaa tggtcttcac ctcagcatcc accttccggg
     4321 acagtcccag ccctccgacc tgctctacat cctggatata cagccccagg ggggccttca
     4381 gtgcttccca cagcctcctg tcaaccctct caaggtggac tgggggctgc ccatccccag
     4441 cccctccccc attcacccgg cccatcacaa gcgggatcgc agacagatct tcctgccaga
     4501 gcccgagcag ccctcgaggc ttcaggatcc agttctcgta agctgcgact cggcgccctg
     4561 tactgtggtg cagtgtgacc tgcaggagat ggcgcgcggg cagcgggcca tggtcacggt
     4621 gctggccttc ctgtggctgc ccagcctcta ccagaggcct ctggatcagt ttgtgctgca
     4681 gtcgcacgca tggttcaacg tgtcctccct cccctatgcg gtgcccccgc tcagcctgcc
     4741 ccgaggggaa gctcaggtgt ggacacagct gctccgggcc ttggaggaga gggccattcc
     4801 aatctggtgg gtgctggtgg gtgtgctggg tggcctgctg ctgctcacca tcctggtcct
     4861 ggccatgtgg aaggtcggct tcttcaagcg gaaccggcca cccctggaag aagatgatga
     4921 agagggggag tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct
     4981 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     5041 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5101 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5161 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5221 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5281 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     5341 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5401 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5461 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5521 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5581 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5641 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5701 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     5761 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5821 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     5881 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     5941 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6001 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     6061 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     6121 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     6181 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6241 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6301 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     6361 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6421 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6481 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6541 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6601 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6661 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     6721 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6781 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6841 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6901 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     6961 ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     7021 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7081 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     7141 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7201 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     7261 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7321 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7381 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7441 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7501 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7561 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7621 taacgcagga aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8101 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8221 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8281 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8341 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8401 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8461 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8521 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8581 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8641 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8701 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8761 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8821 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8881 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8941 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9001 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9061 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9121 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9181 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9241 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9301 tcatactctt cctttttc
//
