LOCUS       sequence-128541-        1797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a theophylline binding riboswitch, 
            controlling the translation initiation rate of luciferase gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-Theo27 pUC19
FEATURES             Location/Qualifiers
     source          1..1797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(13..18,19..34,35..41)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             122..1774
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(188..206)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggatccgagc tgttgacaat taatcatcgg ctcgtataat gtgtgggacg ctactcatct
       61 tttaggtgat accagcatcg tcttgatgcc cttggcagca ccagctgcta ggccgttcaa
      121 gatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg
      181 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      241 tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc
      301 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      361 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      421 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat
      481 ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca
      541 aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg
      601 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
      661 ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg
      721 atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc
      781 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
      841 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
      901 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta
      961 caaaattcaa agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct
     1021 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc
     1081 gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata
     1141 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     1201 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     1261 aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg
     1321 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     1381 tggagacata gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc
     1441 tttaattaaa tacaaaggat atcaggtggc ccccgctgaa ttggaatcga tattgttaca
     1501 acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact
     1561 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     1621 ttacgtggcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     1681 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     1741 aaaggccaag aagggcggaa agtccaaatt gtaaccgcac accttactgg tgtgcgg
//
