LOCUS       sequence-128554-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CDK1-mCherry in pIVT vector for in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Davydenko O, Schultz RM, Lampson MA
  TITLE     Increased CDK1 activity determines the timing of 
            kinetochore-microtubule attachments in meiosis I.
  JOURNAL   J Cell Biol. 2013 Jul 22;202(2):221-9. doi: 10.1083/jcb.201303019. 
            Epub 2013 Jul 15.
  PUBMED    23857768
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201303019"; journalName: "J Cell Biol"; date: 
            "2013-07-22- 22"; volume: "202"; issue: "2"; pages: "221-9"
COMMENT     Sequence Label: Cdk1-mCherry
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..743)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(311..330)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     580..598
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tagtcagtca ctagaagggc gaattcgagc tcttacttgt acagctcgtc catgccgccg
       61 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
      121 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
      181 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
      241 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
      301 cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
      361 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
      421 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
      481 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
      541 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
      601 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
      661 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
      721 tcctcctcgc ccttgctcac catggatcct ctagagtcga ccatcttctt aatctgattg
      781 tccaagtcat c
//
