LOCUS       sequence-128563-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses zebrafish Chk1 mutant with C-term GFP fluorescent tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Zhang M, Kothari P, Mullins M, Lampson MA
  TITLE     Regulation of zygotic genome activation and DNA damage checkpoint 
            acquisition at the mid-blastula transition.
  JOURNAL   Cell Cycle. 2014;13(24):3828-38. doi: 10.4161/15384101.2014.967066.
  PUBMED    25558827
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2014"; volume: "13(24):3828-38. doi"; pages: " 
            10.4161/15384101.2014.967066"
COMMENT     Sequence Label: pMZ031
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(32..745,746..748,749..751))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(35..745,746..748,749..751))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     426..445
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tacgactcac tatagggctg cagaatctag attacttgta cagctcgtcc atgccgagag
       61 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      121 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
      181 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
      241 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
      301 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
      361 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      421 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      481 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
      541 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      601 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
      661 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      721 cggtgaacag ctcctcgccc ttgctcacca tctcgagaat caatggcaaa accttttggt
      781 tgctaataat gtcagacagc ttctgtttga gtttc
//
