LOCUS       sequence-128568-        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      386..395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             392..1102
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 aaccgtcaga tccgctagcg ccaccatgaa gttaagtcgc cagttcaccg tgtttggcag
       61 cgcgatcttc tgcgtcgtaa tcttctcact ctacctgatg ctggacaggg gtcacttgga
      121 ctaccctcgg ggcccgcgcc aggagggctc ctttccgcag ggccagcttt caatattgca
      181 agaaaagatt gaccatttgg agcgtttgct cgctgagaac aacgagatca tctcaaatat
      241 cagagactca gtcatcaacc tgagcgagtc tgtggaggac ggcccgcggg ggtcaccagg
      301 caacgccagc caaggctcca tccacctcca ctcgccacag ttggccctgc aggctgaccc
      361 cagctccgga tcggatccac cggtcgccac catggtgtct aagggcgaag agctgatcaa
      421 ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg
      481 cacaggtgaa ggagaaggca atccgtacat gggaactcaa accatgagga tcaaagtcat
      541 cgagggagga cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag
      601 ccgtactttt atcaagtacc cgaaaggcat tcctgatttc tttaaacagt cctttcctga
      661 gggttttact tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcatgca
      721 ggacaccagc cttgaggatg gctgtctcgt ttaccacgtc caagtcagag gggtaaactt
      781 tccctccaat ggtcccgtga tgcagaagaa gaccaagggt tgggagccta atacagagat
      841 gatgtatcca gcagatggtg gtctgagggg atacactcat atggcactga aagttgatgg
      901 tggtggccat ctgtcttgct ctttcgtaac aacttacagg tcaaaaaaga ccgtcgggaa
      961 catcaagatg cccggtatcc atgccgttga tcaccgcctg gaaaggttag aggaaagtga
     1021 caatgaaatg ttcgtagtac aacgcgaaca cgcagttgcc aagttcgccg ggcttggtgg
     1081 tgggatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc agccat
//
