LOCUS       sequence-128570-        2509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2509)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Paxillin-KM-N-18
FEATURES             Location/Qualifiers
     source          1..2509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1753..1762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1759..2469
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1904..1922)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2172..2191
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc caccatgatg gacgacctcg atgccttact ggcagacctg
       61 gaatccacca cctcgcatat ctctaagcga ccggtgtttc taacagagga aacgccttac
      121 tcctacccaa ctggaaacca tacgtaccag gagattgctg tgccaccgcc ggtgcccccg
      181 ccgccttcca gtgaggccct aaatggcact gtgattgacc ccttagacca gtggcaaccc
      241 agcgtgtcca gatacggcca ccagcaacct cagtcccagt ctccgatata cagctctagt
      301 gccaaaagct ccagtgcctc tgttcctaga gacgggctca gctccccttc tccccgtgcc
      361 agtgaggagg aacacgtgta cagcttccca aacaagcaga agtctgcaga accatctccc
      421 tcactgacca gcacctccct gggcagcaac ctctcggaac tggacaggct tctcctggaa
      481 ctgaatgctg ttcaacataa tccccccagt ggcttctcag cagatgaagt cagcagaagc
      541 ccatcactgc ccaatgtgac cggacctcac tatgtcatcc cagagagcag cagctctgca
      601 ggagggaagg ctgcaccccc tacaaaagaa aaaccaaagc ggaatggtgg gcgtgggatt
      661 gaagatgtgc gtcccagtgt ggagagcctg ctggatgagc tggagagctc tgtgccaagc
      721 ccagtccctg caatcactgt gagccaaggg gaggtgagca gcccccagcg tgtcaacgcc
      781 agtcagcagc agacccgtat ctctgcttct tcagctacac gagaactgga tgagctgatg
      841 gcgtccctct ctgactttaa gttcatggca caggggaaag ccggcggcag cagctcccca
      901 ccatccacca ccccaaagcc tggcagtcag ctggacacca tgctgggaag tctgcagtct
      961 gacctgaaca aactgggggt agcgacggtc gccaagggtg tgtgcggagc ctgcaagaag
     1021 ccgattgctg ggcaggtagt tacagccatg gggaagacct ggcaccctga gcacttcgtc
     1081 tgcacccact gccaggagga gattggatca cggaacttct ttgagcggga tgggcagccc
     1141 tactgtgaga aggactatca caacctcttc tctcctcgct gctactactg caatgggcca
     1201 atccttgata aagtggtgac ggctttggac aggacgtggc accctgaaca ctttttctgt
     1261 gcccagtgtg gagttttctt tggacctgaa ggatttcatg agaaggatgg caaagcctat
     1321 tgccgcaagg actactttga catgtttgct cccaagtgtg gaggctgtgc ccgggctatc
     1381 ctggaaaatt acatttctgc cttaaacacc ctgtggcacc ctgagtgctt tgtctgtcgg
     1441 gaatgtttca caccgttcat caacggcagc ttcttcgagc acgacgggca gccctactgc
     1501 gaggtgcatt accacgagcg ccgcggctcg ctgtgctctg gctgccagaa gcccatcacg
     1561 ggacgctgca tcactgccat gggcaagaaa tttcaccccg aacactttgt ctgtgccttc
     1621 tgcctcaagc agctcaacaa aggaaccttc aaagaacaga acgacaagcc ctactgtcag
     1681 aactgctttc tcaaactctt ctgtagcagc ggtggagcaa gcgcagccag tggtagcgcg
     1741 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     1801 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1861 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1921 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1981 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     2041 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2101 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     2161 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     2221 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     2281 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2341 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2401 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     2461 tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacatt
//
