LOCUS       sequence-128603-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to knockdown endogenous RAB14 expression levels.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Kim M, Tan YS, Cheng WC, Kingsbury TJ, Heimfeld S, Civin CI
  TITLE     MIR144 and MIR451 regulate human erythropoiesis via RAB14.
  JOURNAL   Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164.
  PUBMED    25312678
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Br J 
            Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164."
COMMENT     Sequence Label: RAB14 shRNA
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(27..537)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(90..108)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    586..703
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 accatggtgg cgaccggtgg atccccctgg ggagagaggt cggtgattcg gtcaacgagg
       61 gagccgactg ccgacgtgcg ctccggaggc ttgcagaatg cggaacaccg cgcgggcagg
      121 aacagggccc acactaccgc cccacacccc gcctcccgca ccgccccttc ccggccgctg
      181 ctctcggcgc gccctgctga gcagccgcta ttggccacag cccatcgcgg tcggcgcgct
      241 gccattgctc cctggcgctg tccgtctgcg agggtactag tgagacgtgc ggcttccgtt
      301 tgtcacgtcc ggcacgccgc gaaccgcaag gaaccttccc gacttagggg cggagcagga
      361 agcgtcgccg gggggcccac aagggtagcg gcgaagatcc gggtgacgct gcgaacggac
      421 gtgaagaatg tgcgagaccc agggtcggcg ccgctgcgtt tcccggaacc acgcccagag
      481 cagccgcgtc cctgcgcaaa cccagggctg ccttggaaaa ggcgcaaccc caaccccctc
      541 gagccgcggc caaagtggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt
      601 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
      661 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
      721 catttgtctc gaggtcgaga attaaaaaag gagcgatt
//
