LOCUS       sequence-128612-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-EGFP-ccdB
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     212..233
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(291..307)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(291..307)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(322..763)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(328..351)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    646..763
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(787..807)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(787..805)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(826..842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(826..842)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(839..861)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    850..866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(874..880,881..898,899..904))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    919..940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
       61 ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
      121 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      181 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      241 accaccccgg tgaacagctc ctcgcccttg ctcaccattt tgtcccgggg gatccactag
      301 ttctagaatc cggggttttt tctccttgac gttaaagtat agaggtatat taacaatttt
      361 ttgttgatac ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta
      421 ttagttaaag tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat
      481 cgcttcgctg attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta
      541 tggttgttaa tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt
      601 cctcttcata accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg
      661 cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt
      721 cttccttcgg agggctgtca cccgctcggc ggcttctaat ccgtactaga gctccagctt
      781 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
      841 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      901 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      961 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1021 g
//
