LOCUS       sequence-128619-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The heterotrimeric G-protein subunit, G-alpha-i isoform 2, tagged 
            with mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: Galphai2-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(742..759)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tgctccctga tgacctgtcc ggcgtcatcc ggaggctctg ggctgaccat ggtgtgcagg
       61 cctgctttgg ccgctcaagg gaataccagc tcaacgactc agctgcctac tacctgaacg
      121 acctggagcg tattgcacag agtgactaca tccccacaca gcaagatgtg ctacggaccc
      181 gcgtaaagac cacggggatc gtggagacac acttcacctt caaggaccta cacttcaaga
      241 tgtttgatgt gggtggtcag cggtctgagc ggaagaagtg gatccactgc tttgagggcg
      301 tcacagccat catcttctgc gtagccttga gcgcctatga cttggtgcta gctgaggacg
      361 aggagatgaa ccgcatgcat gagagcatga agctattcga tagcatctgc aacaacaagt
      421 ggttcacaga cacgtccatc atcctcttcc tcaacaagaa ggacctgttt gaggagaaga
      481 tcacacacag tcccctgacc atctgcttcc ctgagtacac aggggccaac aaatatgatg
      541 aggcagccag ctacatccag agtaagtttg aggacctgaa taagcgcaaa gacaccaagg
      601 agatctacac gcacttcacg tgcgccaccg acaccaagaa cgtgcagttc gtgtttgacg
      661 ccgtcaccga tgtcatcatc aagaacaacc tgaaggactg cggcctcttc tgaagcttaa
      721 taagtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      781 gcccctcccc cgtgccttcc ttgaccctgg aa
//
