LOCUS       sequence-128622-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The heterotrimeric G-protein subunit, G-alpha-i isoform 2, tagged 
            with mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: Galphai2-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(532..553)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(793..812)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cgggccctct agactcgagc ggccgccact gtgctggata
      121 tctgcagaat tcaccatggg ctgcaccgtg agcgccgagg acaaggcggc ggccgagcgc
      181 tctaagatga tcgacaagaa cctgcgggag gacggagaga aggcggcgcg ggaggtgaag
      241 ttgctgctgt tgggtgctgg ggagtcaggg aagagcacca tcgtcaagca gatgaagatc
      301 atccacgagg atggctactc cgaggaggaa tgccggcagt accgggcggt tgtctacagc
      361 aacaccatcc agtccatcat ggccattgtc aaagccatgg gcaacctgca gatcgacttt
      421 gccgacccct ccagagcgga cgacgccagg cagctatttg cactgtcctg caccgccacc
      481 ggttctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      541 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      601 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      661 cccaccctcg tgaccaccct gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac
      721 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      781 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      841 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      901 gggcacaagc tggagtacaa ctactt
//
