LOCUS       sequence-128623-         336 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoterless AAV plasmid with MCS to insert promoter for expression 
            of EmGFP. Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2157
FEATURES             Location/Qualifiers
     source          1..336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(41..60)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          43..175
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 cgcccttgct caccatggtg gcggccgcct gggagtggac acctgtggag agaaaggcaa
       61 agtggatgtc agtaagacca ataggtgcct atcagaaacg caagagtctt ctctgtctcg
      121 acaagcccag tttctattgg tctccttaaa cctgtcttgt aaccttgata cttacctgcc
      181 cagtgcctca cgaccaactt cggcgcgcca cgcgtggccg gcctagggaa ttccgatctt
      241 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
      301 cctagtgatg gagttggcca ctccctctct gcgcgc
//
