LOCUS       sequence-128627-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG416GPD-EYFP-ccdB
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(292..308)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(292..308)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(314..958)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(397..420)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(983..1003)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(983..1001)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1022..1038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1022..1038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1035..1057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1046..1062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 ggcgaagcac tgcaggccgt agccgaaggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatt ttgtcccggg ggatccacta
      301 gttctagaat ccgtcgaaac taagttctgg tgttttaaaa ctaaaaaaaa gactaactat
      361 aaaagtagaa tttaagaagt ttaagaaata gatttacaga attacaatca atacctaccg
      421 tctttatata cttattagtc aagtagggga ataatttcag ggaactggtt tcaacctttt
      481 ttttcagctt tttccaaatc agagagagca gaaggtaata gaaggtgtaa gaaaatgaga
      541 tagatacatg cgtgggtcaa ttgccttgtg tcatcattta ctccaggcag gttgcatcac
      601 tccattgagg ttgtgcccgt tttttgcctg tttgtgcccc tgttctctgt agttgcgcta
      661 agagaatgga cctatgaact gatggttggt gaagaaaaca atattttggt gctgggattc
      721 tttttttttc tggatgccag cttaaaaagc gggctccatt atatttagtg gatgccagga
      781 ataaacctgt tcacccagac acctacgatg ttatatattc tgtgtaaccc gccccctatt
      841 ttgggcatgt acgggttaca gcagaattaa aaggctaatt ttttgactaa ataaagttag
      901 gaaaatcact actattaatt atttacgtat tctttgaaat ggcgagtatt gataatgata
      961 aactgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     1021 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattcc
//
