LOCUS       sequence-128629-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pCaSpeR4 mito roGFP2-Orp1
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..225
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(292..366)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
       61 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      121 ggtggagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      181 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      241 ggtgaacagc tcctcgccct tgctcacgga tcccggcggc aacgaatgga tcttggcgcg
      301 cggcactggg agccgccggg ccgagcctgt caagccccgc agcagcagcg gcgtcaggac
      361 ggacatgcgg ccgcggatct gatccccggg cgggtacctt cacgctgtgg atgaggagga
      421 agggaaaacg gatgattagt tacacacccc aagaaaatcg ataattgcag agccgcgccc
      481 aacttgagcc ggcactgcgt gcgagtgaga gaggtagcta aatggtatac tgattttttt
      541 gctctacttt gccgacgccg gaattttact ctctcgccat tttctcttta attctatcca
      601 aaatctacag ttatgcgtcg aagtatcatg gtaaccgaat ttagaatttt taagattaat
      661 gaagcgttcc agcagcatag aaataatgaa tat
//
