LOCUS       sequence-128630-       12071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 12071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pCaSpeR4 mito roGFP2-Orp1
FEATURES             Location/Qualifiers
     source          1..12071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..83
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(108..110,111..821)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(150..171)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(411..430)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     758..779
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1573..1654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1610..1629)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1664..1683
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(1718..1919,1920..1950)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(2487..2506)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2606..2628
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2666..2684)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2752..2856
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2857..2925,2926..3717)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3075..3094)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3888..4476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4377..4396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(4711..4741,4742..5296)
                     /label=P element 5' end
     gene            complement(5307..9442)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     9415..9433
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gcggccgcat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc tcggcccggc
       61 ggctcccagt gccgcgcgcc aagatccatt cgttgccgcc gggatccgtg agcaagggcg
      121 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      181 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      241 agttcatctc caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      301 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      361 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      421 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      481 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      541 acaactgcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
      601 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      661 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacctgct
      721 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      781 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gactagtggt ggttcaggtg
      841 gtggtggttc aggtggtggt ggttcaggtg gaggaggatc aggaggagga ggatcaggag
      901 gaggaggatc aggaggagaa ttcgatatct cagaattcta taagctagca cctgttgaca
      961 agaaaggcca accattcccc ttcgaccaat taaagggaaa agtggtgctt atcgttaatg
     1021 ttgcctccaa atgtggattc actcctcaat acaaagaact agaggccttg tacaaacgtt
     1081 ataaggacga aggatttacc atcatcgggt tcccatgcaa ccagtttggc caccaagaac
     1141 ctggctctga tgaagaaatt gcccagttct gccaactgaa ctatggcgtg actttcccca
     1201 ttatgaaaaa aattgacgtt aatggtggca atgaggaccc tgtttacaag tttttgaaga
     1261 gccaaaaatc cggtatgttg ggcttgagag gtatcaaatg gaattttgaa aaattcttag
     1321 tcgataaaaa gggtaaagtg tacgaaagat actcttcact aaccaaacct tcttcgttgt
     1381 ccgaaaccat cgaagaactt ttgaaagagg tggaatagtc tagagtcgac ctcgaacgtt
     1441 aacgttaacg taacgttaac tcgagctaga tcataatcag ccataccaca tttgtaaagg
     1501 ttttaccgcg agaaaaaacc tcccacacct ccccctgaac ctgaaacata acttgtctgc
     1561 gttgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1621 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1681 atcaatgtat gtcgaggtcg acctgcagcc caagctttgc gtactcgcaa attattaaaa
     1741 ataaaacttt aaaaataatt tcgtctaatt aatattatga gttaattcaa accccacgga
     1801 catgctaagg gttaatcaac aatcatatcg ctgtctcact cagactcaat acgacactca
     1861 gaatactatt cctttcactc gcacttattg caagcatacg ttaagtggat gtctcttgcc
     1921 gacgggacca ccttatgtta tttcatcatg gtctggccat tctcatcgtg agcttccggg
     1981 tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc
     2041 gaaataactg cttgtttttt tttttaccat tattaccatc gtgtttactg tttattgccc
     2101 cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga cctatagaat
     2161 acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct tctaaccaaa
     2221 agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg
     2281 gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac
     2341 cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca
     2401 ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gtgcggtatt
     2461 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2521 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2581 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2641 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     2701 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2761 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2821 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2881 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     2941 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3001 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3061 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3121 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3181 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3241 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3301 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3361 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3421 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3481 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3541 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3601 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3661 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3721 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3781 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3841 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3901 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3961 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4021 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4081 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4141 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4201 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4261 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4321 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4381 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4441 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccttcttctt gaactcgggc
     4501 tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag
     4561 ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg
     4621 aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc
     4681 gatctgccgc tggactacgt gggtctggcc catgatgaaa taacataagg tggtcccgtc
     4741 gatagccgaa gcttaccgaa gtatacactt aaattcagtg cacgtttgct tgttgagagg
     4801 aaaggttgtg tgcggacgaa tttttttttg aaaacattaa cccttacgtg gaataaaaaa
     4861 aaatgaaata ttgcaaattt tgctgcaaag ctgtgactgg agtaaaatta attcacgtgc
     4921 cgaagtgtgc tattaagaga aaattgtggg agcagagcct tgggtgcagc cttggtgaaa
     4981 actcccaaat ttgtgatacc cactttaaag attcgcagtg gaaggctgca cctgcaaaag
     5041 gtcagacatt taaaaggagg cgactcaacg cagatgccgt acctagtaaa gtgatagagc
     5101 ctgaaccaga aaagataaaa gaaggctata ccagtgggag tacacaaaca gagtaagttt
     5161 gaatagtaaa aaaaatcatt tatgtaaaca ataacgtgac tgtgcgttag gtcctgttca
     5221 ttgtttaatg aaaataagag cttgagggaa aaaattcgta ctttggagta cgaaatgcgt
     5281 cgtttagagc agcagccgaa ttaattctag ttccagtgaa atccaagcat tttctaaatt
     5341 aaatgtattc ttattattat agttgttatt tttgatatat ataaacaaca ctattatgcc
     5401 caccattttt ttgagatgca tctacacaag gaacaaacac tggatgtcac tttcagttca
     5461 aattgtaacg ctaatcactc cgaacaggtc acaaaaaatt accttaaaaa gtcataatat
     5521 taaattagaa taaatatagc tgtgagggaa atatatacaa atatattgga gcaaataaat
     5581 tgtacataca aatatttatt actaatttct attgagacga aatgaaccac tcggaaccat
     5641 ttgagcgaac cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg
     5701 tgaatgtgtt gcggagagcg ggtgggagac agcgaaagag caactacgaa acgtggtgtg
     5761 gtggaggtga attatgaaga gggcgcgcga tttgaaaagt atgtatataa aaaatatatc
     5821 ccggtgtttt atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct
     5881 tttggttaga agacaaatcc aaagtctact tgtggggatg ttcgaagggg aaatacttgt
     5941 attctatagg tcatatcttg tttttattgg cacaaatata attacattag ctttttgagg
     6001 gggcaataaa cagtaaacac gatggtaata atggtaaaaa aaaaaacaag cagttatttc
     6061 ggatatatgt cggctactcc ttgcgtcggg cccgaagtct tagagccaga tatgcgagca
     6121 cccggaagct cacgatgaga atggccagac ccacgtagtc cagcggcaga tcggcggcgg
     6181 agaagttaag cgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg
     6241 tgcagctaat ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg
     6301 cgtaacggaa ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt
     6361 tcaagaagaa gccgccaaag agcaggaatg gtatgataac cggcggaccc acagacagcg
     6421 ccatcgaggt cgaggagctg gcgcaggata ttagatatcc gaaggacgtt gacacattgg
     6481 ccaccagagt gaccagcgcc aggcagttga agaagtgcag cactccggcc cgcagtccga
     6541 tcatcggata ggcaatcgcc gtgaagacca gtggcactgt gagaaaaagc ggtaattcgg
     6601 caatcgtttt gcccagaaag tatgtgtcac agcgataaag tcgacttcgg gcctccctca
     6661 taaaaactgg cagctctgag gtgaacacct aaatcgaatc gattcattag aaagttagta
     6721 aattattaat atgcaaatgt attctaaaca agacttacat ttatcgtggc aaagacgttt
     6781 tgaaaggtca tgttggtcag gaagaggaag atggctccgt tgatattcat cacgcccact
     6841 tgcgtgagtt gttggcccaa aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta
     6901 aaatgttact cgcatctcat taatattcat atcttcaaca tgttcgcgag ttaaatgaaa
     6961 tttatttatt ttctgcaaaa ctataaacta tacatctcat tgaaaaaaac taagaagggt
     7021 gtggaatcag gcaattctaa ctaaaatcta gcgaatttgt ttccaagaat tgtaagcgtt
     7081 atatcatttg tttccactgg aaccactcac cgttgtctga ataagtcgca cttttacgag
     7141 gagtggttcc ttgagcaccg acagccagga tcgccacagg accgcccgga actgcatgaa
     7201 ccaggtggcc ttgtaggtgt acccattctc cggctgctcc agtggcttct ccaaattttt
     7261 ggtggccaac aactgctcca tatcccgggc tactttgcta atagcaaaat tgtcgccata
     7321 tcttggcgat ccgatcacgg gactcgatct cccgtccggg cacaacggcc aacacctgta
     7381 cgtaaaagtc cgccggattg tagttggtag gacactgggc acccacgctg gataggagtt
     7441 gagatgtaat gtaatgctag atacccttaa taaacacatc gaactcacta ggaaaagaag
     7501 tcgacggctt cgctgggagt gcccaagaaa gctaccctgc cctcggccat cagaaggatc
     7561 ttgtcaaaga gctcaaacag ctcggaagac ggctgatgaa tggtcaggat gacggtcttg
     7621 cccttctgcg acagcttctt cagcacctgg acgacgctgt gggcggtaaa tgagtccagt
     7681 ccggaggtgg gctcatcgca gatcagaagc ggcggatcgg ttagtgcctc ggaggcgaat
     7741 gccagacgct tcctttctcc gccggacaga cctttcaccc tgccgggcac accgatgatc
     7801 gtgtgctgac atttgctgag cgaaagctcc tggatcacct gatccacgcg ggccactcgc
     7861 tgccgatagg tcagatgtcg tggcatccgc accatggcct ggaaaatcag gtgttccctg
     7921 gccgttaggg agccgataaa gaggtcatcc tgctggacat aggcgcacct ggcctgcatc
     7981 tccttggcgt ccacaggttg gccattgagc agtcgcatcc cggatggcga tacttggatg
     8041 ccctgcggcg atcgaaaggc aagggcattc agcagggtcg tctttccggc accggaactg
     8101 cccatcacgg ccaaaagttc gcccggatag gccacgccgc aaactgagtt tcaaattggt
     8161 aattggaccc tttattaaga tttcacacag atcagccgac tgcgaataga aactcaccgt
     8221 tcttgagcaa atgtttcctg ggcgccggta tgtgtcgctc gttgcagaat agtccgcgtg
     8281 tccggttgac cagctgccgc catccggagc ccggctgatt gaccgcccca aagatgtcca
     8341 tattgtgcca ggcataggtg aggttctcgg ctagttggcc gctccctgaa ccggagtcct
     8401 ccggcggact gggtggcagg agcgtgccgt agtttttggc ctgcccgaag ccctggttaa
     8461 tgcagctctg cgaagcgtcc gctgtcaccc tgcaatgata ggggatctca aatatcaact
     8521 acaagcgtta tgctcatcta accccgaaca aaacgaagta tcctacgaag taggtttata
     8581 cttttattta ttttttgtgc atctaggatc agcttaaaat atctggttgt tatatttttt
     8641 gtaaaaaaga atgtagtcga aaatgaatgc ctttagatgt cttgatcatg atatgatctt
     8701 aaaaattgtc ttatatagcg agcacagcta ccagaataat ctgtttcgtg tcactatttg
     8761 tttgtgcgat tgcggtttgg gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg
     8821 atgttgcaat cgcagttcct atagatcaag tgaacttaag atgtatgcac atgtactact
     8881 cacattgttc agatgctcgg cagatgggtg tttgctgcct ccgcgaatta atagctcctg
     8941 atcctcttgg cccattgccg ggatttttca cactttcccc tgcttaccca cccaaaacca
     9001 atcaccaccc caatcactca aaaaacaaac aaaaataaga agcgagagga gttttggcac
     9061 agcactttgt gtttaattga tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac
     9121 aaagtgcaac tgaaggcgga cattgacgct aggtaacgct acaaacggtg gcgaaagaga
     9181 tagcggacgc agcggcgaaa gagacggcga tatttctgtg gacagagaag gaggcaaaca
     9241 gcgctgactt tgagtggaat gtcattttga gtgagaggta atcgaaagaa cctggtactt
     9301 caaataccct tggatcgaag taaatttaaa actgatcaga taagttcaat gatatccagt
     9361 gcagtaaaaa ataaaaaaaa aatatgtttt tttaaatcta cattctccaa aaaagggttt
     9421 tattaactta catacatact agaattcgat atcaagcttg cacaggtcct gttcgataac
     9481 gtcgtactcg ggaatcacct ttaacttgtc cgctattggg tatttgtcct taaacacatt
     9541 cgtaatctct cccacaatca ttgccctggc ctctgcaatg taaaaagtat ttataaaata
     9601 tcgctggctc taaagaaaac atattaatca aagtattgag gctttatcaa tctaacattt
     9661 acaagtagtc agaccacaat ttttagactg cacttctgcg taagtaaatg aaatgaaatg
     9721 gtgacctact cttgggtagc cgtactgtta tatgctgacg agccttcacc tgggcctgct
     9781 ttttctcctg tttcgtctga tgcgtcggat gcggctggac tggctgattg ttgggattgg
     9841 gttttggatg ctgctccgaa aggtgggcca cagcttttgc gatggtggtg cccactatgc
     9901 ggttgctgca ggaactacag ctgaatgttc gctttattgt ctgcagctcc tggggctgga
     9961 cgaaggccac gccctcaccc ggtgcggcca tctcctgcgg caaaacgtat gtgggattcc
    10021 tgtccaagta gctctcgatg cccaccataa tgcgctccag caggttggcc aggtgcgggt
    10081 ctagctgcgg cggattcgtc gtctggttca ctgagtttag ggtctgcaga aagaagtgtg
    10141 cctccatggt aagcggattc atctgcacga tcagcgggct ttagtctaaa aattcgaatg
    10201 atacaaactc gtactcacgt aaagcagttt gtttggcatc atctcggcca tcgtcttatt
    10261 cttcttcgtg gcctttgtta ccggagcggc tatgttgggc gcggtggagc ccggaagctg
    10321 aaaggttaat cgaatgcgtt aacttttctg caactcgaaa gtttgccgcc tttgttcgac
    10381 tgccaataac tgttgattcg aaaattcgaa tcgaagcggt tgaatttcgt agggtggcca
    10441 actacaccaa agttcgccgg cggtgtatgc atgtgagtgt gagtgtgaca gtgggtgtac
    10501 gtgtgcttta agcgacacaa tcgcaccgcg atgtacatat ttttgtagaa atgtgtgtgc
    10561 atttttcgtc cggatcacgg ctccataaaa taaatcatat cacagggctc agggatttgt
    10621 gagtagaagt tcccgccact taaacgtcac ttgtgaaatc gccatcgacg gtcaactttt
    10681 actcacactg gtcttccgcg gacggaccgt ctcaaagtac tgcctttctg cgttggaaaa
    10741 catcgccttt ttcgtccaaa aggagtcccc aggttcgatc cgcatggcgt tgtgcgtgcg
    10801 tgcctttctt ttcaaatgat tacggctatt aacttggggg cgttaagttg gaaacacgta
    10861 aattgcagac tgcgattaga gtgaccatga gtaggagttc aaaatctcct gacatcattt
    10921 tcttaaaacc tgctttgttt tttacatttc tatttaatat aactcctatt tgaataaaaa
    10981 aacaaaacaa gtttagatgt taagatatta actacatcct ttgctccaaa gggagagggg
    11041 aagttatgga gttaattaat ttgctgttgg aaatcaatat ggagtcagaa atataatgat
    11101 ttactaaacc ttattgaatc ggtaacgatg cgaatttata ttaaaatagc ttttatgaaa
    11161 cattcaacaa aaatattatt aatgttggcc cactttagca accggttagg tctaccggtt
    11221 gggcaagcaa agattcacgc cctggttcga gtcccaacta gtcctgcaaa ataccgcagc
    11281 aagttttaga gagaccaagt gccattacct ctcccacttc agttatcggt tatgcggcgt
    11341 ttaagtcgac agcttgccgt ctctagctcc ggtgcctata taaagcagcc cgctttccac
    11401 atttcatatt cgttttacgt ttgtcaagcc tcatagccgg cagttcgaac gtatacgctc
    11461 tctgagtcag acctcgaaat cgtagctcta cacaattctg tgaattttcc ttgtcgcgtg
    11521 tgaaacactt ccaataaaaa ctcctgcagg tgagtacttt aaaaaaaaat ctagtgaaat
    11581 aatgctgaaa agaaatttgt gtgggcaaaa ttcaatgggc aaaaacgcga tgcggctttt
    11641 tctcaaaatg gcggccggcc tgcgtttttt cctcaaaagt gatgacgtca tgcctgtttt
    11701 ttttttttgt tcgcaatgag gaatggctct taaaatctac tagataaaaa aaatattcat
    11761 tatttctatg ctgctggaac gcttcattaa tcttaaaaat tctaaattcg gttaccatga
    11821 tacttcgacg cataactgta gattttggat agaattaaag agaaaatggc gagagagtaa
    11881 aattccggcg tcggcaaagt agagcaaaaa aatcagtata ccatttagct acctctctca
    11941 ctcgcacgca gtgccggctc aagttgggcg cggctctgca attatcgatt ttcttggggt
    12001 gtgtaactaa tcatccgttt tcccttcctc ctcatccaca gcgtgaaggt acccgcccgg
    12061 ggatcagatc c
//
