LOCUS       sequence-128631-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses diffuse K560-mCherry-eDHFR .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Ballister ER, Ayloo S, Chenoweth DM, Lampson MA, Holzbaur EL
  TITLE     Optogenetic control of organelle transport using a photocaged 
            chemical inducer of dimerization.
  JOURNAL   Curr Biol. 2015 May 18;25(10):R407-8. doi: 
            10.1016/j.cub.2015.03.056.
  PUBMED    25989077
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2015.03"; journalName: "Curr Biol"; date: 
            "2015-05-18- 18"; volume: "25"; issue: "10"; pages: "R407-8"
COMMENT     Sequence Label: pERB258
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(73..102)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(103..576)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 caccagccac caccttctga taggcagcct gcacctgagg agtgaattgc ggccgcctcg
       61 agcgcttaat taaagatcct cttctgagat gagtttttgt tcccgccgct ccagaatctc
      121 aaagcaatag ctgtgagagt tctgcgcatc agcatcgtgg aattcgctga ataccgattc
      181 ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg cgtcgatatg
      241 cgtcagatac agtttttgcg cttttggcaa gaactgttca taaacgcgac cgccgccaat
      301 caccatgatt tctggtacgt caccacacgc cgcgatggct tcatccaccg acttcaccca
      361 cgttacgcga tcgtccgtac ccggttgact gctgaggata atatttttgc gtcctggcaa
      421 cggacgaccg attgattccc aggtatggcg gcccataatc acgggtttat ttaaggtgtt
      481 gcgtttaaac caggcgagat cggcaggcag gttccacggc atggcgtttt ccatgccgat
      541 aacgcgatct accgctaacg ccgcaatcag actgattcta gactccaggc cggccttata
      601 cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgat
      661 ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt aggcgccggg
      721 cagctgcacg ggcttcttgg ccttgta
//
