LOCUS       sequence-128633-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses KLC1-mCherry-eDHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Ballister ER, Ayloo S, Chenoweth DM, Lampson MA, Holzbaur EL
  TITLE     Optogenetic control of organelle transport using a photocaged 
            chemical inducer of dimerization.
  JOURNAL   Curr Biol. 2015 May 18;25(10):R407-8. doi: 
            10.1016/j.cub.2015.03.056.
  PUBMED    25989077
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2015.03"; journalName: "Curr Biol"; date: 
            "2015-05-18- 18"; volume: "25"; issue: "10"; pages: "R407-8"
COMMENT     Sequence Label: pERB257
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..97)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(98..571)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 gccaccacct tctgataggc agcctgcacc tgaggagtga attgcggccg cctcgagcgc
       61 ttaattaaag atcctcttct gagatgagtt tttgttcccg ccgctccaga atctcaaagc
      121 aatagctgtg agagttctgc gcatcagcat cgtggaattc gctgaatacc gattcccagt
      181 catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca
      241 gatacagttt ttgcgctttt ggcaagaact gttcataaac gcgaccgccg ccaatcacca
      301 tgatttctgg tacgtcacca cacgccgcga tggcttcatc caccgacttc acccacgtta
      361 cgcgatcgtc cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac
      421 gaccgattga ttcccaggta tggcggccca taatcacggg tttatttaag gtgttgcgtt
      481 taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc
      541 gatctaccgc taacgccgca atcagactga ttctagactc caggccggcc ttatacagct
      601 cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt
      661 agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct
      721 gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgta
//
