LOCUS       sequence-128634-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The heterotrimeric G-protein subunit, G-alpha-i isoform 3, tagged 
            with mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: Galphai3-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(492..513)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(753..772)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagcg tttaaactta agcttggtac ccaccatggg ctgcacgttg agcgccgaag
      121 acaaggcggc agtggagcga agcaagatga tcgaccgcaa cttacgggag gacggggaaa
      181 aagcggccaa agaagtgaag ctgctgctac tcggtgctgg agaatctggt aaaagcacca
      241 ttgtgaaaca gatgaaaatc attcatgagg atggctattc agaggatgaa tgtaaacaat
      301 ataaagtagt tgtctacagc aatactatac agtccatcat tgcaatcata agagccatgg
      361 gacggctaaa gattgacttt ggggaagctg ccagggcaga tgatgcccgg caattatttg
      421 ttttagctgg cagtgctacc ggttctatgg tgagcaaggg cgaggagctg ttcaccgggg
      481 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      541 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      601 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct
      661 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      721 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      781 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      841 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctactttagc gacaacgtct
      901 atatcaccgc cgacaagcag aagaacggca tcaaggccaa ct
//
