LOCUS       sequence-128645-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             53..748
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 agcagagctg gtttgtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag
       61 cgagctgatt aaggagaaca tgcccatgaa gctgtacatg gagggcaccg tgaacaacca
      121 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
      181 aatcaaggtc gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
      241 catgtacggc agcagaacct tcatcaagca ccctccgggc atccccgact tctttaagca
      301 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
      361 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaaggttag
      421 aggggtgaac ttcccagcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
      481 ctccaccgag acgatgtacc ccgctgacgg cggcctggaa ggcgcatgtg acatggccct
      541 gaagctcgtg ggcgggggcc acctgatctg caaccttgag accacataca gatccaagaa
      601 acccgctacg aacctcaaga tgcccggcgt ctacaacgtg gaccacagac tggaaagaat
      661 caaggaggcc gacgatgaga cctacgtcga gcagcacgag gtggctgtgg ccagatactc
      721 tactggtggc gctggtgatg gaggtaaagg tggaggaggt tccggactca gatctggcag
      781 cagtggaggt tctgggggca aatacgtaga ctcggaggga catctctaca ccgttcccat
      841 ccgggaacag ggcaacatct acaagcccaa caacaaggcc atggcagacg agctgagcga
      901 gaagcaagtg tacgacgcgc acaccaagga gatcgacctg gtcaaccgcg accctaaaca
      961 cctcaacgat gac
//
