LOCUS       sequence-128646-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-PlastinC-10
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..742
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcgagct
       61 gattaaggag aacatgccca tgaagctgta catggagggc accgtgaaca accaccactt
      121 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
      181 ggtcgtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
      241 cggcagcaga accttcatca agcaccctcc gggcatcccc gacttcttta agcagtcctt
      301 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
      361 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaagg ttagaggggt
      421 gaacttccca gccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac
      481 cgagacgatg taccccgctg acggcggcct ggaaggcgca tgtgacatgg ccctgaagct
      541 cgtgggcggg ggccacctga tctgcaacct tgagaccaca tacagatcca agaaacccgc
      601 tacgaacctc aagatgcccg gcgtctacaa cgtggaccac agactggaaa gaatcaagga
      661 ggccgacgat gagacctacg tcgagcagca cgaggtggct gtggccagat actctactgg
      721 tggcgctggt gatggaggta aaggtggagg aggttccgga agcggctccg ggagcggaag
      781 cggggaaaac agtactacta ccatttctcg ggaggagctt gaagaactac aagaggcatt
      841 taataaaata gatattgaca atagt
//
