LOCUS       sequence-128648-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR with FRT sites + pT7::LacO::SgrS::T1 terminator + ColE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Shin JE, Lin C, Lim HN
  TITLE     Horizontal transfer of DNA methylation patterns into bacterial 
            chromosomes.
  JOURNAL   Nucleic Acids Res. 2016 Apr 15. pii: gkw230.
  PUBMED    27084942
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 15. pii: gkw230."
COMMENT     Sequence Label: pHL 2147
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(373..406)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     441..460
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        441..459
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    462..481
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
       61 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
      121 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
      181 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
      241 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
      301 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgagctt
      361 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcgaac tgcaggtcga
      421 cggatccccg gaatgacgtc taatacgact cactataggg gaattgtgag cgctcacaat
      481 tcccgggatg aagcaagggg gtgccccatg cgtcagtttt atcagcacta ttttaccgcg
      541 acagcgaagt tgtgctggtt gcgttggtta agcgtcccac aacgattaac catgcttgaa
      601 ggactgatgc agtgggatga ccgcaattct gaaagttgac ttgcctgcat catgtgtgac
      661 tgagtattgg tgtaaaatca cccgccagca gattatacct gctggttttt tttattctcg
      721 ccgcgctaaa aagcttgggc ccatggtacg cgtgctagag gcatcaaata a
//
