LOCUS       sequence-128650-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PS-CFP2-mPlum-FRET-10
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..760
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     regulatory      785..794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatga gcaagggcgc
       61 cgagctgttc accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca
      121 caagttcagc gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      181 gttcatctgc accaccggca agctgcctgt gccctggccc accctggtgg ccaccctgag
      241 ctacggcgtg cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa
      301 gagcgccatg cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa
      361 ctacaagacg cgcgccgagg tgaagttcga gggcgatacc ctggtgagtc gcatcgagct
      421 gaccggcact gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta
      481 caacgccacc aatgtgtaca tcgtggccga caaggccagg aatggcatca aggtgaactt
      541 caaggtccgc cacaacatca aggatggcag cgtgcagctg gccgaccact accagcagaa
      601 tacccccatc ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag
      661 cgccctgtcc aaggacccca acgagaagcg cgatcacatg atctacctcg agttcgtgac
      721 cgccgccgcc atcacccacg gcatggatga gctgtacaag tccggactca gatccccacc
      781 ggtcgccacc atggtgagca agggcgagga ggtcatcaag gagttcatgc gcttcaagga
      841 gcacatggag ggctccgtga a
//
