LOCUS       sequence-128651-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral empty control vector, CMV promoter, Blast .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV Blast empty (w263-1)
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(188..212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(296..316)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(714..725)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
       61 tctagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc
      121 caccatggga accaattcag tcgactggat ccggtaccga attcgcggcc gcactcgaga
      181 tatctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
      241 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
      301 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
      361 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
      421 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
      481 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
      541 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
      601 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
      661 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
      721 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
      781 ttcgccttcg ccctcag
//
