LOCUS       sequence-128653-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The heterotrimeric G-protein subunit, G-alpha-i isoform 3, tagged 
            with mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: Galphai3-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(755..772)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    761..872
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagtcatgac tccagaacta gcaggagtga ttaaacggtt atggcgagat ggtggggtac
       61 aagcttgctt cagcagatcc agggaatatc agctcaatga ttctgcttca tattatctaa
      121 atgatctgga tagaatatcc cagtctaact acattccaac tcagcaagat gttcttcgga
      181 cgagagtgaa gaccacaggc attgtagaaa cacatttcac cttcaaagac ctatacttca
      241 agatgtttga tgtaggtggc caaagatcag aacgaaaaaa gtggattcac tgttttgagg
      301 gagtgacagc aattatcttc tgtgtggccc tcagtgatta tgaccttgtt ctggctgagg
      361 acgaggagat gaaccgaatg catgaaagca tgaaactgtt tgacagcatt tgtaataaca
      421 aatggtttac agaaacttca atcattctct tccttaacaa gaaagacctt tttgaggaaa
      481 aaataaagag gagtccgtta actatctgtt atccagaata cacaggttcc aatacatatg
      541 aagaggcagc tgcctatatt caatgccagt ttgaagatct gaacagaaga aaagatacca
      601 aggagatcta tactcacttc acctgtgcca cagacacgaa gaatgtgcag tttgtttttg
      661 atgctgttac agatgtcatc attaaaaaca acttaaagga atgtggactt tattaacctc
      721 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      781 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      841 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      901 ctgggg
//
