LOCUS       sequence-128674-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  deleted OPTN was inserted into pEGFP-N1 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Turturro S, Shen X, Shyam R, Yue BY, Ying H
  TITLE     Effects of mutations and deletions in the human optineurin gene.
  JOURNAL   Springerplus. 2014 Feb 19;3:99. doi: 10.1186/2193-1801-3-99. 
            eCollection 2014.
  PUBMED    24683533
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Springerplus."; date: "2014-02-19"; volume: "3:99. doi"; pages: " 
            10.1186/2193-1801-3-99. eCollection 2014"
COMMENT     Sequence Label: pOPTN1-55-EGFP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(318..341)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(432..453)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     781..800
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta tctagatccg
      121 gtggatcccg ggcccgcggt accgtcgact tatttcagct ggtggttctc ggtcaggagc
      181 tctttcatct gctgcagcag ctcctccggg gtaaacgtgt ccaggtttgg gtgggccagg
      241 tgggggggtc catttcctgt gctttcactg gggctgtcct ccttttcagt gaggcagctg
      301 agaggttgat gggacatctt atcgtcgtca tccttgtaat ccatggtgga atcacgcgtc
      361 tcgaggctag caatggatct gagtccggac ttgtacagct cgtccatgcc gagagtgatc
      421 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      481 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      541 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
      601 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
      661 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      721 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      781 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgg
//
