LOCUS       sequence-128680-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on 
            CAG expression vector. DDdCas9VP192 protein is stabilised by 
            Trimethoprim..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski 
            T
  TITLE     Conditionally Stabilized dCas9 Activator for Controlling Gene 
            Expression in Human Cell Reprogramming and Differentiation.
  JOURNAL   Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 
            10.1016/j.stemcr.2015.08.001.
  PUBMED    26352799
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.08"; journalName: "Stem Cell Reports"; date: 
            "2015-09-8- 8"; volume: "5"; issue: "3"; pages: "448-59"
COMMENT     Sequence Label: CAG-DDdCas9VP192-T2A-EGFP-ires-puro
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..237
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(289..342)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(382..492)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             complement(544..654)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             complement(706..816)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             complement(868..978)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             complement(1015..1035)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
       61 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
      121 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      181 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      241 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atatcgattg ggccaggatt
      301 ctcctcgacg tcaccgcatg ttagcagact tcctctgccc tctccggagc cgtcgacgct
      361 agcgctgggt agtgacccgg ggagcatgtc aaggtcaaaa tcgtcaagag cgtcagcagg
      421 cagcatatca aggtcaaagt cgtcaagggc atcggctggg agcatgtcta agtcaaaatc
      481 gtcaagggcg tcggccggcc cgccgctttc gcactcgacg ctagcgctgg gtagtgaccc
      541 ggggagcatg tcaaggtcaa aatcgtcaag agcgtcagca ggcagcatat caaggtcaaa
      601 gtcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg cgtcggccgg
      661 cccgccgctt tcgcactcga cgctagcgct gggtagtgac ccggggagca tgtcaaggtc
      721 aaaatcgtca agagcgtcag caggcagcat atcaaggtca aagtcgtcaa gggcatcggc
      781 tgggagcatg tctaagtcaa aatcgtcaag ggcgtcggcc ggcccgccgc tttcgcactc
      841 gacgctagcg ctgggtagtg acccggggag catgtcaagg tcaaaatcgt caagagcgtc
      901 agcaggcagc atatcaaggt caaagtcgtc aagggcatcg gctgggagca tgtctaagtc
      961 aaaatcgtca agggcgtcgg ccggcccgcc gctttcgcac tcgaggctgg cctccacctt
     1021 tctcttcttc ttggggctgt cgcctcc
//
