LOCUS       sequence-128695-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GAL-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..164)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(172..188)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(172..188)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(203..644)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(209..232)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    527..644
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(668..688)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(707..723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(707..723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(720..742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    731..747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(755..761,762..779,780..785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    800..821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
       61 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      121 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtgatggg ggatccacta
      181 gttctagaat ccggggtttt ttctccttga cgttaaagta tagaggtata ttaacaattt
      241 tttgttgata cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt
      301 attagttaaa gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca
      361 tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct
      421 atggttgtta atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt
      481 tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc
      541 gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag
      601 tcttccttcg gagggctgtc acccgctcgg cggcttctaa tccgtactag agctccagct
      661 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
      721 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      841 ccgctttcca gtcgggaaa
//
