LOCUS       sequence-128698-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG303GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     83..101
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(139..161)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     261..280
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        427..614
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
       61 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
      121 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
      181 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
      241 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accataaatt
      301 cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt gaacacggca
      361 ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta ttactcttgg
      421 cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat tttttttttt
      481 ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat aatgaattat
      541 acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag caggcaagat
      601 aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca aatgaaacca
      661 agattcagat tgcgatctct ttaaagggtg gtcccctagc gatagagcac tcgatcttcc
      721 cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg attaacgtcc
      781 acacaggtat agggtttctg gaccatatga tacatgctct ggccaagcat tccggctggt
      841 cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc actgaagact
      901 gcgggattgc tctcggtcaa gcttttaaa
//
