LOCUS       sequence-128700-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG303GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..169)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(177..193)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(177..193)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(199..842)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(282..305)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(862..881)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(863..881)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt gactggatat
       61 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
      121 tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtg atgggggatc
      181 cactagttct agaatccgtc gaaactaagt tctggtgttt taaaactaaa aaaaagacta
      241 actataaaag tagaatttaa gaagtttaag aaatagattt acagaattac aatcaatacc
      301 taccgtcttt atatacttat tagtcaagta ggggaataat ttcagggaac tggtttcaac
      361 cttttttttc agctttttcc aaatcagaga gagcagaagg taatagaagg tgtaagaaaa
      421 tgagatagat acatgcgtgg gtcaattgcc ttgtgtcatc atttactcca ggcaggttgc
      481 atcactccat tgaggttgtg cccgtttttt gcctgtttgt gcccctgttc tctgtagttg
      541 cgctaagaga atggacctat gaactgatgg ttggtgaaga aaacaatatt ttggtgctgg
      601 gattcttttt ttttctggat gccagcttaa aaagcgggct ccattatatt tagtggatgc
      661 caggaataaa ctgttcaccc agacacctac gatgttatat attctgtgta acccgccccc
      721 tattttgggc atgtacgggt tacagcagaa ttaaaaggct aattttttga ctaaataaag
      781 ttaggaaaat cactactatt aattatttac gtattctttg aaatggcgag tattgataat
      841 gataaactga gctccaattc gccctatagt gagtcgtatt acaattcact ggcc
//
