LOCUS       sequence-128704-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid for gRNA concatenation Golden Gate cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski 
            T
  TITLE     Conditionally Stabilized dCas9 Activator for Controlling Gene 
            Expression in Human Cell Reprogramming and Differentiation.
  JOURNAL   Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 
            10.1016/j.stemcr.2015.08.001.
  PUBMED    26352799
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.08"; journalName: "Stem Cell Reports"; date: 
            "2015-09-8- 8"; volume: "5"; issue: "3"; pages: "448-59"
COMMENT     Sequence Label: GG-dest
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(129..134,135..152,153..159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    167..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     191..207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        226..244
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     464..486
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     478..495
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     479..495
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        505..523
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agctctaata cgactcacta
      241 tagggagaca agcttgcatg cctgcaggtc gactctagag gatctcgagc tcaagcttcg
      301 aattccccct ggagacgggc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
      361 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      421 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      481 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg
      541 ccccccctcg aggtcct
//
