LOCUS       sequence-128705-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep 
            expression construct. Note that an improved version is available: 
            Addgene plasmid# 92307. See comments for details.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Zinn E, Pacouret S, Khaychuk V, Turunen HT, Carvalho LS, 
            Andres-Mateos E, Shah S, Shelke R, Maurer AC, Plovie E, Xiao R, 
            Vandenberghe LH
  TITLE     In Silico Reconstruction of the Viral Evolutionary Lineage Yields a 
            Potent Gene Therapy Vector.
  JOURNAL   Cell Rep. 2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 
            10.1016/j.celrep.2015.07.019.
  PUBMED    26235624
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 
            10.1016/j.celrep.2015.07.019."
COMMENT     Sequence Label: pAnc80L65
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     123..139
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     123..139
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(617..628)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaggca ccccaggctg tggaatttca
      121 cacaggaaac agctatgacc atgattacgc caagcgcgcg aacaaaagct ggagctccac
      181 cgcggtggcg gccgctctag tcgagcggcc gccagtgtga tggatatctg cagaattcgg
      241 cttggcggct gcgcgttcaa acctcccgct tcaaaatgga gaccctgcgt gctcactcgg
      301 gcttaaatac ccagcgtgac cacatggtgt cgcaaaatgt cgcaaaacac tcacgtgacc
      361 tctaatacag gacctctaga gcatggaaac tagataagaa agaaatacgc agagaccaaa
      421 gttcaactga aacgaattaa acggtttatt gattaacaag caaactagtt tacagattac
      481 gggtgaggta acgggtgccg atggggcgag gctcagaata aacgccattt gtgtcaacag
      541 caaagtccac atttgtagat ttgttgtagt tggaagtgta ttgaatctct gggttccagc
      601 gtttgctgtt ttctttctgc agctcccatt caatttccac gctgacctgt ccggtgctgt
      661 actgcgtgat gaacgacgca aacttagctg gactgaaggt agttggagga ttcgcgggaa
      721 caggtgtatt cttaatcagg atctgaggag gcgggtgttt cagtccaaag cctcccatca
      781 gcggcgaggg atgaaagtgt ccgtccgtgt gaggaatctt ggcccagata ggaccctgca
      841 ggtacacgtc ccggtcctgc cagaccatgc caggtaaggc tccttgactg ttgacggtcc
      901 ctgtagcagg agcggtgttg gccgattgca ggttagtggc caccgtgccg tactcttctg
      961 tggccactgg gttggtggtt ttaatttctt cctcgttggt tatcataacg ttgtcaaggt
     1021 ccacgttgct atttccagct ccctgtttcc caaatattaa gactccgctc atcggaaaaa
//
