LOCUS       sequence-128712-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An empty sgRNA expression vector for expression of sgRNA for Sp 
            Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Pham H, Kearns NA, Maehr R
  TITLE     Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian 
            Cells.
  JOURNAL   Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3.
  PUBMED    26463376
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1358:43-57. doi"; pages: " 
            10.1007/978-1-4939-3067-8_3"
COMMENT     Sequence Label: pLenti SpBsmBI sgRNA Hygro
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        225..300
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(579..623)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
       61 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      121 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      181 tatcttgtgg aaaggacgaa acaccgagac gattaatgcg tctcgtttta gagctagaaa
      241 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      301 tttttttcta gtgttaaccg ataccgtcga gatccgttca ctaatcgaat ggatctgtct
      361 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
      421 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
      481 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
      541 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
      601 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
      661 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
      721 atctgttcca gagatttatt actccaacta gcattcc
//
