LOCUS       sequence-128714-         309 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An empty sgRNA expression vector for expression of sgRNA for Sp 
            Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Pham H, Kearns NA, Maehr R
  TITLE     Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian 
            Cells.
  JOURNAL   Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3.
  PUBMED    26463376
REFERENCE   2  (bases 1 to 309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1358:43-57. doi"; pages: " 
            10.1007/978-1-4939-3067-8_3"
COMMENT     Sequence Label: pLenti SpBsmBI sgRNA Hygro
FEATURES             Location/Qualifiers
     source          1..309
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttcacaaat tttgtaatcc agaggttgat tgtcgagatt tgtgtacgcc cgacagtccc
       61 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
      121 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
      181 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
      241 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
      301 catcgcctc
//
