LOCUS       sequence-128751-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2229
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(58..91)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(154..351)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cgactcacta tagggcgaat tgggtgcata ggccactagt ggatctgata tcacctaata
       61 acttcgtata gcatacatta tacgaagtta tattaagggt tctcgagagc tcgttttcga
      121 cactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg
      181 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag
      241 gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa
      301 aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tactgattat
      361 aaaccatcat tttcgatgaa ataatcaatc aacgtagata agctgatatt atataatttt
      421 ggtctgttcg tgttgatttt atcactgatg gactttggca tacagatagt gacaatttcg
      481 ttattgaacc attgagaatg gaaaatcaat ggaacttcat ccagagttat gcacatagaa
      541 gctccctcag ccggaaaaaa gctgatagcg ccaaaatcta ttagtgacaa gtctgtgtta
      601 aggccagttc cagtaaattt tgtatacgac tccttcaagg accataagta agtaaatatt
      661 gtgcatggat cagacgcttt cagtaaaccg ttaaattctc tttcactaaa aacttcttta
      721 aatagctcca actcttccct cccgccataa ttgcacggag aagcgatatc aattccgaca
      781 tcctggtatt catctgtact tacacatttt acgaggaaca tagctacata ttgttcaccg
      841 atggtcatgc taaatggaag aaaacgattg ttgtctaaga atggcttacc gaagctgccc
      901 ttgtcaaatt tcagctcttg aaaatttaag cccgttacta tagagcagcc aaacaactgc
      961 agcagctggc tgcatagatt tgaacatcta t
//
