LOCUS       sequence-128755-        3467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-nasF 
            attenuator-MCSII, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 57
FEATURES             Location/Qualifiers
     source          1..3467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      136..222
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        317..397
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     rep_origin      1011..1469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1098..1117)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1311..1332
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      complement(1579..2167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1659..1678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2338..3129,3130..3198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2961..2980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3199..3303)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3371..3389
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3437..3456
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3437..3455
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgcgc gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
       61 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
      121 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      181 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
      241 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
      301 gaatttgata gagataagtc cagccttgca agaagcggat acaggagtgc aaaaaatggc
      361 tatctctaga aaggcctacc ccttaggctt tatgcaacag aaacaataat aatggagtca
      421 tgaccatgac aatgcacctg gggctcgact atatagatag tctcgttgaa gaagatgaga
      481 acgagggcat ctaccgctgc aagcgcgaga tgttcaccga ccctcggctg ttcgatttag
      541 agatgaaaca catctttgag ggcaactgga tttatctcgc ccacgagagc cagattcccg
      601 agaagaacga ctattacacc acgcagatgg gccggcagcc gatattcatc acacgcaaca
      661 aagatggtga gctgaatgcc ttcgtcaatg cctgaattcg agtgaataaa aggttttggg
      721 cagcgcgcca atggcggcgc gtatgtccag ggataaaggc gtccagcggt gcgtaagcac
      781 cgccgggcgc tttttttttg cgcgaattcc caattcggtt cacccgggca tatggcaaga
      841 actgcagata tccatggtac cgagctcgga tccgtcgaca agcttgcggc cgcccagctt
      901 gctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct
      961 gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg
     1021 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1081 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1141 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1201 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc ggccatcgcc
     1261 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     1321 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     1381 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     1441 ttttaacaaa atattaacgt ttacaatttc taaccgatcc ccctggagct tggaatcagg
     1501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1981 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2581 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2641 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2701 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2761 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2821 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2881 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2941 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3001 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3061 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3301 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3361 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattgg tcgcatcgat
     3421 gctggcttat cgaaattaat acgactcact atagggagac cggaatt
//
