LOCUS       sequence-128756-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-nasF 
            attenuator-MCSII, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 57
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(688..768)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     terminator      complement(863..949)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
       61 gctacagggc gcgtactatg gttgctttga cgagcacgta taacgtgctt tcctcgttag
      121 aatcagagcg ggagctaaac aggaggccga ttaaagggat tttagacagg aacggtacgc
      181 cagcaagctg ggcggccgca agcttgtcga cggatccgag ctcggtacca tggatatctg
      241 cagttcttgc catatgcccg ggtgaaccga attgggaatt cgcgcaaaaa aaaagcgccc
      301 ggcggtgctt acgcaccgct ggacgccttt atccctggac atacgcgccg ccattggcgc
      361 gctgcccaaa accttttatt cactcgaatt caggcattga cgaaggcatt cagctcacca
      421 tctttgttgc gtgtgatgaa tatcggctgc cggcccatct gcgtggtgta atagtcgttc
      481 ttctcgggaa tctggctctc gtgggcgaga taaatccagt tgccctcaaa gatgtgtttc
      541 atctctaaat cgaacagccg agggtcggtg aacatctcgc gcttgcagcg gtagatgccc
      601 tcgttctcat cttcttcaac gagactatct atatagtcga gccccaggtg cattgtcatg
      661 gtcatgactc cattattatt gtttctgttg cataaagcct aaggggtagg cctttctaga
      721 gatagccatt ttttgcactc ctgtatccgc ttcttgcaag gctggactta tctctatcaa
      781 attccctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa
      841 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
      901 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag
      961 ttccctactc tcgcatggg
//
