LOCUS       sequence-128758-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF
            attenuator-T7 SD sequence-MCSII, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 58
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             261..283
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(722..802)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     terminator      complement(897..983)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
       61 gctacagggc gcgtactatg gttgctttga cgagcacgta taacgtgctt tcctcgttag
      121 aatcagagcg ggagctaaac aggaggccga ttaaagggat tttagacagg aacggtacgc
      181 cagcaagctg ggcggccgca agcttgtcga cggatccgag ctcggtacca tggatatctg
      241 cagttcttgc catatgtata tctccttctt aaagttaaac aaaattattt cccgggtgaa
      301 ccgaattggg aattcgcgca aaaaaaaagc gcccggcggt gcttacgcac cgctggacgc
      361 ctttatccct ggacatacgc gccgccattg gcgcgctgcc caaaaccttt tattcactcg
      421 aattcaggca ttgacgaagg cattcagctc accatctttg ttgcgtgtga tgaatatcgg
      481 ctgccggccc atctgcgtgg tgtaatagtc gttcttctcg ggaatctggc tctcgtgggc
      541 gagataaatc cagttgccct caaagatgtg tttcatctct aaatcgaaca gccgagggtc
      601 ggtgaacatc tcgcgcttgc agcggtagat gccctcgttc tcatcttctt caacgagact
      661 atctatatag tcgagcccca ggtgcattgt catggtcatg actccattat tattgtttct
      721 gttgcataaa gcctaagggg taggcctttc tagagatagc cattttttgc actcctgtat
      781 ccgcttcttg caaggctgga cttatctcta tcaaattccc tggcagttta tggcgggcgt
      841 cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc cggcggattt
      901 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
      961 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat gg
//
