LOCUS       sequence-128759-        3501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF
            attenuator-T7 SD sequence-MCSII, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 58
FEATURES             Location/Qualifiers
     source          1..3501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      136..222
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        317..397
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             836..858
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     rep_origin      1045..1503
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1132..1151)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1345..1366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      complement(1613..2201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1693..1712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2372..3163,3164..3232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2995..3014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3233..3337)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3405..3423
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3471..3490
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3471..3489
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgcgc gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
       61 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
      121 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      181 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
      241 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
      301 gaatttgata gagataagtc cagccttgca agaagcggat acaggagtgc aaaaaatggc
      361 tatctctaga aaggcctacc ccttaggctt tatgcaacag aaacaataat aatggagtca
      421 tgaccatgac aatgcacctg gggctcgact atatagatag tctcgttgaa gaagatgaga
      481 acgagggcat ctaccgctgc aagcgcgaga tgttcaccga ccctcggctg ttcgatttag
      541 agatgaaaca catctttgag ggcaactgga tttatctcgc ccacgagagc cagattcccg
      601 agaagaacga ctattacacc acgcagatgg gccggcagcc gatattcatc acacgcaaca
      661 aagatggtga gctgaatgcc ttcgtcaatg cctgaattcg agtgaataaa aggttttggg
      721 cagcgcgcca atggcggcgc gtatgtccag ggataaaggc gtccagcggt gcgtaagcac
      781 cgccgggcgc tttttttttg cgcgaattcc caattcggtt cacccgggaa ataattttgt
      841 ttaactttaa gaaggagata tacatatggc aagaactgca gatatccatg gtaccgagct
      901 cggatccgtc gacaagcttg cggccgccca gcttgctggc gtaccgttcc tgtctaaaat
      961 ccctttaatc ggcctcctgt ttagctcccg ctctgattct aacgaggaaa gcacgttata
     1021 cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1081 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     1141 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     1201 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     1261 tgggtgatgg ttcacgtagt gggcggccat cgccctgata gacggttttt cgccctttga
     1321 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1381 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1441 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     1501 tttctaaccg atccccctgg agcttggaat caggggataa cgcaggaaag aacatgtgag
     1561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2041 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     2701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     3001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3361 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3421 ggccctttcg tcttcaagaa ttggtcgcat cgatgctggc ttatcgaaat taatacgact
     3481 cactataggg agaccggaat t
//
