LOCUS       sequence-128763-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-H2
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(27..58)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggggcgta aacgccatgg gcatgtagtc aaaagcctcc ggtcggaggc ttttgacttg
       61 gctgaggagt gccgttaatt aagttcactc atctttcagg gcttttatcg ccggatgtaa
      121 atcaacagat tgtaacagac cacccggcgg cgttttcaca agttgcggac ataaccgttc
      181 cacgtccgcc agcagggtta tggcctgtgc cattgaccac tgcgcatgtt cgctcatcaa
      241 atttcgtgca atccactgcg caatattgcc aggttcgaat acggactgct tcgccaggta
      301 gcctatcagt tcaggaatca agatttgtaa attttgttgg cgtaagggta aaggcactgc
      361 cctgatggtc acatgctgtg catctgactg gaaatcaata cccaattccg ccagggcaga
      421 ctgcgctttt tctaatgccg atttttcttc ggcagaaact tttagccgca acggaatcag
      481 cagcggctgg gcgcaaacgg gcgcttcacc cggcgtcaat tgtgcctgac gcagccaacg
      541 ttctgccact ggcaaggata aaagtgaaat gttgccgtcg cgctccagca acgcacagtc
      601 ggaatggacg atagtcagta cccgaccaaa actctgactg ttcgccgcaa gtgcaggttc
      661 ctgcggttcc ggcgctttta atttttgcat c
//
