LOCUS       sequence-128765-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-J
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(44..75)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(749..768)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ctgcgatgag tggcagggcg gggcgtaaac gccatgggca tgtagtcaaa agcctccggt
       61 cggaggcttt tgacttggct gaggagtgcc gttaattaag tttaaacgta ccttccagca
      121 tcaacttcca gccaggttgg cttttcacgc tgctcagcca gctccagagc ggctttcacg
      181 cgagactgct tcttcgcttt ctcacgaatg cttacaacgt cattcggact aacctgataa
      241 gaagcgatgt taacaacacg accgtttacc ataattgctt tatggctaac cagctgacgt
      301 gcttctgcac gagtggcacc gaagcccata cggtatacaa cgttgtccag acgaccttcc
      361 agcagagcca acaggttttc accggtgttg cctttcagac gtgctgcttc tttgtagtag
      421 ttacggaact gacgctccag cacaccatag atacggcgaa ctttttgctt ttcacgcaac
      481 tgcacaccat agtcagacag acgcggttta cgcgcaccgt gctggccagg agcttgttca
      541 attttacact tggtatcgat cgcgcgaacg ccagacttaa ggaataagtc ggtgccctca
      601 cgacggctca gcttgagctt aggacccaaa tatcttgcca tagcggtcat ttatgtcaga
      661 cttgagttgt acgcgtccaa aaaaacgggt atggagaaac agtagagagt tgcgataaaa
      721 agcgtcaggt aggatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga
      781 cgccgtgcaa ataatcaatg tggactt
//
