LOCUS       sequence-128768-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-R4
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(37..68)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(95..691)
                     /codon_start=1
                     /gene="AICDA"
                     /product="human activation-induced cytidine deaminase 
                     (Dickerson et al., 2003)"
                     /label=AID
                     /translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
ORIGIN
        1 gagtggcagg gcggggcgta aacgccatgg gcatgtagtc aaaagcctcc ggtcggaggc
       61 ttttgacttg gctgaggagt gccgttaatt aagtttacag acccagggta cggaacgcgt
      121 cacgcaggtc gtcaacttcg tacagcggca gcaggatacg acgcagctga cgagacagac
      181 gaacagagtt ttcgtgcaga ccttcccacg ctttgaaggt acgttcgtgg ttttcaacga
      241 aggtgttcca gcagtagaag tagtctttga aggtcatgat cgcgatctga acacccgcac
      301 ggtgcagacg acgcagacct tccggttccg ctttacggtc ttcgcagaag tacagacgcg
      361 cggtgaagat acgcagagac aggttcgggt taccacgcag gaagtccgca acgtgacgcg
      421 cgcagtcgta gcacggagac caagaggtga accaggtaac acggtagcaa cgacccgggt
      481 ccaggtccca gtcagagatg taacgcagga acagcagttc aacgtggcaa ccgtttttgt
      541 tacgcaggta accgaagtcc agagagaaag aggtcgcaga gtcacgacgt ttaacaacgt
      601 agcacaggta ggtttcacga cgacctttcg cccaacgaac gtttttgaac tggtacagga
      661 atttgcgacg gttcatcagc agagagtcca tcttcagatt ccggtt
//
