LOCUS       sequence-128770-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked kanMX marker, integration into S. cerevisiae X-3 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2223
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(58..91)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(154..351)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cgactcacta tagggcgaat tgggtgcata ggccactagt ggatctgata tcacctaata
       61 acttcgtata gcatacatta tacgaagtta tattaagggt tctcgagagc tcgttttcga
      121 cactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg
      181 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag
      241 gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa
      301 aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tactgattag
      361 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
      421 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
      481 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
      541 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
      601 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
      661 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
      721 tgagcgagac gaaatacacg gtcgctgtta aaaggacaat tacaaacagg aatcgaatgt
      781 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
      841 tctaatacct ggaatgctgt tttgccgggg atcgcagtgg tgagtaacca tgcatcatca
      901 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtc
//
