LOCUS       sequence-128771-         601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-T3
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(17..48)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aacgccatgg gcatgtagtc aaaagcctcc ggtcggaggc ttttgacttg gctgaggagt
       61 gccgttaatt aagtttacag acccagggta cggaacgcgt cacgcaggtc gtcaacttcg
      121 tacagcggca gcaggatacg acgcagctga cgagacagac gaacagagtt ttcgtgcaga
      181 ccttcccacg ctttgaaggt gatgccgtgg ttttcaacga aggtgttcca gcagtagaag
      241 tagtctttga aggtcatgat cgcgatctga acacccgcac ggtgcagacg acgcagacct
      301 tccggttccg cacgacggtc ttcgcagaag tacagacgcg cggtgaagat acgcagagac
      361 aggttcgggt taccacgcag gaagtccgca acgtgacgcg cgcagtcgta gcacggagac
      421 caagagatga accaggtaac acggtagcaa cgacccgggt ccaggtccca gtcagagatg
      481 taacgcagga acagcatttc aacgtggcaa ccgtttttgt tacgcaggta accgaagtcc
      541 agagagaaag aggtcgcaga gtcacggctt tcaacaacgt agcacaggta ggtttcacga
      601 c
//
