LOCUS       sequence-128789-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of MBP-TEV n-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T
  TITLE     APP controls the formation of PI(3,5)P vesicles through its binding 
            of the PIKfyve complex.
  JOURNAL   Cell Mol Life Sci. 2015 Jul 28.
  PUBMED    26216398
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2015 Jul 28."
COMMENT     Sequence Label: pET28-MBP-TEV
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(658..678)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(697..720)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      828..837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagcg
       61 gttagggagc ctttgccccg agggggctaa cttcttcttt cctttctgag tcctcatcaa
      121 gactcctgct ggggcagaat gtgattcttt tcttggcata ctcttctatg gagctggata
      181 tggcctccag ccagtcgtct ctttctgcgg cagaactagc tgagaggatg aaagaacgct
      241 ctacactttc aatctttaac tcgttctgat atgcctcctg ggtaggtttt ctgaccttca
      301 ttccagccag cgagagcatg ttgttcagtt tgtacatccc agactgcatg ggtgtggtgt
      361 agagaagagc atcgttaaac aggaaaaaca tccggggctg catgactttc cgagacagct
      421 tcatcagggt gccttcttta agaaaaaccc gcccaggttg cacaatttca tggtgtccac
      481 ttaagctgta ctgaatctgc atgagcttct gaaagttgtc cccttgcttc atggtgtcat
      541 tggcatggtt agctacctct atgacaacag ccagggcatc ttgggtgtct ctgtgatcta
      601 cagagtcttc tagaaggttc tttaagtaat ctgtcagtag cagcctgtac tgagggatcc
      661 ttggaagtat agattttcac ccttggtgat acgagtctgc gcgtctttca gggcttcatc
      721 gacagtctga cgaccgctgg cggcgttgat caccgcagta cgcacggcat accagaaagc
      781 ggacatctgc gggatgttcg gcatgatttc acctttctgg gcgttttcca tggtggcggc
      841 aatacgtgga tctttcgcca actcttcctc gtaagacttc agcgctacgg cacccagcgg
      901 tttgtcttta ttaaccgctt ccagaccttc atcagtcagc agatagtttt cgaggaactc
      961 tttcgccagc tctttgttcg gactggcggc gttaatacct gcgctcagca cgccaacgaa
     1021 cggtttggat ggttgaccct tgaaggtcgg c
//
